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ABSTRACT
| Title |
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Determination of midazolam in rabbit plasma by high performance liquid chromatography with diode array detection |
| Authors |
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Kate O’Hara, Janice Johnstone, Jennifer Schneider, Anthony Quail, David Cottee |
| Keywords |
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HPLC; midazolam, solid phase extraction, small volume assay |
| Issue Date |
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Mar 2020 |
| Abstract |
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Developing HPLC assays that avoid the use of dangerous solvents for drug extraction and require only small volumes of blood are important improvements to assay methodology. This paper describes a simple midazolam assay which uses solid phase extraction with strong cation exchange solid phase extraction cartridges instead of liquid-liquid extraction with organic solvents and commonly available equipment to measure midazolam concentrations in only 300uL of plasma. The small volume of sample required for this makes it useful for application in small animal and neonate clinical studies. |
| Page(s) |
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27-31 |
| ISSN |
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0975-9492 |
| Source |
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Vol. 11, No.03 |
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