Abstract of INTERNATIONAL JOURNAL OF PHARMA SCIENCES AND RESEARCH (IJPSR)

ABSTRACT

Title

Use of A Very Low Dose of Streptozotocin To Induce Diabetic Retinopathy in Rats

Authors

IRAWATI IRFANI, ARIEF S. KARTASASMITA, RIA BANDIARA, A.PURBA

Keywords

very low-dose streptozotocin,diabetic retinopathy, nerve fiber layer, ganglion cell

Issue Date

Mar 2019

Abstract

Background:Diabetic retinopathy is chronic disease involving retinal neuronal structural changes and retinal vascular system disorders. Animal models were modified in order to study the changes in diabetic retinal layer. Variable methods were used on animal models to induce diabetes with Streptozotocin. The purpose of this study was to determinewhethermultiple, very low-dose streptozotocin (STZ) administration would induce diabetic retinopathy in rats,and be associated with a lowermortality rate than the conventionalSTZ injection model of diabetes.Methods:Twenty-four male Wistar rats were divided into a control group (I), a multiple low-dose STZ group (II), and a single, high-dose STZ group (III). Their blood glucose concentrations were measured regularly. After 6 weeks, only rats from groups I and II survived. The thickness of their retinal layers and theirretinal ganglion cell (RGC) morphology were then analyzed.Results: Hyperglycemia was present in groups II and III bythe third day after STZ injection. Rats in group II survived and demonstrated stable high blood glucose concentrations during the 6 weeks of the study. Thinning of the retinal nerve fiber layer (group I: 2.30±0.911 µm vs.group II: 1.17±0.394 µm, p = 0.010) and derangement of the retinal ganglion cellsby score parameter were found in the low-dose STZ group(group I: 2.50vs. group II 1.00, p = 0.010)Conclusion: Multiple lowdoses of STZ were effective at inducingearly diabetic retinopathy. Thinning of the nerve fiber layer and derangement of the ganglion cell layer were detected 6 weeks after injection.

Page(s)

90-96

ISSN

0975-9492

Source

Vol. 10, No.3