ISSN : 0975-9492


Open Access

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Title : Isolation and HPLC Quantification of Berberine Alkaloid from Alpinia galanga and Alpinia calcarata
Authors : Suresh V Nampoothiri, T. Esakkiduraic, K. Pitchumania
Keywords : Berberine, Alkaloid, HPLC, Alpinia galanga, Alpinia calcarata
Issue Date : Jun 2017
Abstract :
Purpose: To isolate and quantify berberine alkaloid for the first time from the rhizomes of Alpinia galanga and Alpinia calcarata. Methods: Isolated from the chloroform fraction of aqueous extract and quantified by high-performance liquid chromatography (HPLC). The purity and the structure of the identified compound (berberine) were characterised by NMR and LC-MS. Result and conclusion: A new, simple, sensitive and selective HPLC method was developed for the quantification of berberine. The developed method was validated according to the International Conference on Harmonization guidelines. The HPLC analysis showed that Alpinia galanga contained an amount of 1340 and Alpinia calcarata had 1355 mg/Kg of berberine. The isolated berberine and its rich fractions of Alpinia galanga and Alpinia calcarata were found to scavenge the DPPH free radical with IC50 values of 22.5, 49.5 and 50.4 g/ ml respectively, though its activity is less than that of a standard polyphenolic compound.
Page(s) : 97-104
ISSN : 0975-9492
Source : Vol. 8, No.06