| Abstract |
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Purpose: The snail Lymnaea acuminata is the intermediate host of liver fluke Fasciola gigantica which caused endemic fasciolosis in the eastern Uttar Pradesh. Aim of the present study is to describes the effect of sublethal treatment (40% and 80% of 4h LC50) of chlorophyllin on the level of different biochemical parameters viz. protein, amino acids, DNA/RNA, acetylcholinesterase, acid and alkaline phosphatase in the nervous tissue of Lymnaea acuminata. Methods: Chlorophyll can be extracted from any green plant. In present study, chlorophyll obtained from spinach was transformed into water-soluble chlorophyllin in 100% ethanol by using different types of chemicals. 20 experimental snails were kept in glass aquarium containing 3l of dechlorinated tap water at 22- 25°C. Snails were exposed to sublethal concentrations 40% and 80% of 4 h LC50 of the chlorophyllin in sunlight. Result: Treatment of 80% of 4 h LC50 of chlorophyllin caused maximum reduction in protein (50.19% of control), amino acid (40.60% of control), DNA (56.14% of control), RNA (51.98% of control) and enzymes acetylcholinesterase (45.06% of control), alkaline phosphatase (56.54% of control) and acid phosphatase (63.17% of control) activity in nervous tissue of L. acuminata. Changes in the biochemical parameters in the nervous tissue of L. acuminata were time and concentration dependent. Withdrawal experiments showed that these changes were reversible. Conclusions: Phytotherapy of snails by photodynamic chlorophyllin is a new approach to control fasciolosis in
developing countries. |
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