ISSN : 0975-9492
CODEN : IJPSQQ





INTERNATIONAL JOURNAL OF PHARMA SCIENCES AND RESEARCH


Open Access

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ABSTRACT

Title : Development and Validation of a Stability indicating HPLCmethod for the Determination of Lamivudine in Bulk and Pharmaceutical dosage form.
Authors : S.V.Saibaba, Dr.P.Shanmuga Pandiyan
Keywords : Lamivudine,RP-HPLC,Validation,Stability indicating, Stress condition
Issue Date : Dec 2016
Abstract :
A simple,selective, precise and accurate RP-HPLC method was developed and validated for the estimation of lamivudine in bulk and pharmaceutical dosage form. The determination was carried out on IntersilC-18 (250 X 4.6mm, 5m) column using a mixture of Acetonitrile,water (50:50% v/v) as mobile phase with a flow rate of 1.0ml/min. The estimation was carried out at 270nm.The method was validated for linearity, accuracy, precision, specificity and robustness as per ICH norms. The retention time of the lamivudine was 2.6 min. The method shows linear responses in the concentration range of 40- 120 g/ml. with correlation coefficient (r2) of 0.999. This method can be useful for the estimation of lamivudine in its pure and tablet dosage forms.
Page(s) : 471-476
ISSN : 0975-9492
Source : Vol. 7, No.12