ISSN : 0975-9492


Open Access

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Title : The Screening Of Cytotoxic Fraction From Elephantopus scaber Linn against Human Cervical Cancer (Hela) Cells
Authors : Nurkhasanah, Kartika Candra Trisnamurti, Retty Dewi Gunaryanti, Trisna Widyastuti, Yeni Listyowati
Keywords : Elephantopus scaber, cytotoxic, IC50, apoptosis.
Issue Date : June 2015
Abstract :
Purpose: Cervical cancer has become the second leading cause of death after breast cancer. Elephantopus scaber Linn (ES) has been used traditionally for curing various diseases. The objective of this study was to explore the active fraction from ES as anticancer and the mode of cell death. Method: The ES herb were extracted using maceration method with ethanol followed by evaporation to get the concentrated extract. The extract were fractionated using petroleum ether, chloroform, ethyl acetate and methanol respectively. The cytotoxic activity of were carried out with MTT method, and the mode of cell death were observed by acridine orange-Ethidium bromide double staining. Result: The result showed that the ES fraction has cytotoxic activity against HeLa cell lines with IC50 values of petroleum ether, chloroform, ethyl acetate and methanol fractions were 185; 42.26; 95.72; 650 g/ml respectively. The mode of cell death showed by the doublestaining method were apoptosis. The cell dead could be distinguish from live cells. The cell dead appear to absorb the ethidium bromide as the DNA of the cell membrane was damage and ethidium bromide could interact with DNA of the cells, while the live cells do not absorb the ethidium bromide as the cell membrane still intact. Conclusion: The chloroform fraction of ES is the most cytotoxic fraction against HeLa cells.
Page(s) : 1011-1014
ISSN : 0975-9492
Source : Vol. 6, No.6