ISSN : 0975-9492
CODEN : IJPSQQ





INTERNATIONAL JOURNAL OF PHARMA SCIENCES AND RESEARCH


Open Access

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ABSTRACT

Title : STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ATENOLOL, LOSARTAN POTASSIUM AND THEIR DEGRADATION PRODUCTS
Authors : Mayur S. Malunjkar, N. B. Mahale, A. D. Landge, S. R. Chaudhari
Keywords : Losartan, Atenolol, RP- HPLC, stress condition, degradation.
Issue Date : April 2015
Abstract :
A simple, sensitive and specific RP-HPLC method was developed for the simultaneous estimation of Atenolol and Losartan potassium (Losartan K) in tablet dosage form. Separation was achieved with an RP C18 (Thermo), 2504.60 mm 5 micron size column, ambient temperature with a low pressure gradient mode with mobile phase containing methanol and 0.1% ortho-phosphoric acid (65:35v/v). The flow rate was 0.9ml/min and eluent was monitored at 274 nm. The selected chromatographic conditions were found to effectively separate Atenolol and Losartan K 4.05 and7.76 min as a retention time respectively. The proposed method was found to be rectilinear over the range of 10-50 g/ml for both Atenolol and Losartan K respectively. The drug was subjected to oxidation, acid hydrolysis, alkaline hydrolysis and heat to apply stress condition for degradation. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability.
Page(s) : 674-682
ISSN : 0975-9492
Source : Vol. 6, No.4