ISSN : 0975-9492


Open Access

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Title : Spectroscopic study on Indacaterol maleate: Analytical applications for quality control of capsules
Authors : Y. A. Salem, D. T. El-Sherbiny, D. R. El-Wasseef, S. M. El-Ashry
Keywords : Indacaterol Maleate (IND), Spectrophotometric, difference Spectrophotometry, 3-methylbenzothiazoline-2-one hydrazone (MBTH), 4-aminoantipyrine (4-AAP), pure form, Capsules.
Issue Date : March 2015
Abstract :
Three simple and sensitive spectrophotometric methods (A, B and C) have been developed for the quantitative estimation of Indacaterol maleate (IND) in bulk drug and capsules. Method A is based on the measurement of the difference absorption spectra of IND in acidic and basic media. The difference spectrum exhibits maxima and minima at 274.6 and 257.8 nm, respectively. Method B is based on the oxidative coupling reaction with an acidic solution of the chromogenic agent 3-methylbenzothiazoline-2-one hydrazone (MBTH) and the drug upon treatment with ceric ammonium sulfate (CAS) produces an orange color peaking at 545 nm. Method C is based on the reaction with 4-aminoantipyrine (4-AAP) in presence of alkaline oxidizing agent; potassium hexacyano ferrate and diluted ammonia (K3 [Fe (CN) 6] / NH3) and measuring the produced red color at 510 nm. Beers law is obeyed in the concentration range of 1.0-16.0 g mL-1, 2.0 20.0 g mL-1 and 3.0 30.0 g mL-1 for Methods A, B and C, respectively. The proposed methods were validated according to ICH guidelines and they were successfully applied for the determination of IND in its capsule dosage forms without interference from excipients.
Page(s) : 592-605
ISSN : 0975-9492
Source : Vol. 6, No.3