ISSN : 0975-9492
CODEN : IJPSQQ





INTERNATIONAL JOURNAL OF PHARMA SCIENCES AND RESEARCH


Open Access

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ABSTRACT

Title : Detection of Melamine Residue in Raw Milk and Milk Related Products by UV spectrophotometry
Authors : T.ANANTHAKUMAR, A.JERAD SURESH, V.NIRAIMATHI
Keywords : UV spectrophotometer, hydrochloric acid, melamine & milk.
Issue Date : February 2015
Abstract :
Melamine is a toxic triazine used as an adulterant in milk & milk related products to increase the protein content. Spectrophotometric method has been developed using 0.1M sodium hydroxide as solvent to detect the melamine residue in raw milk and milk related products. As the amount of melamine residue present in samples would be of nanogram level which is beyond detection by UV spectrophotometer, standard addition method was adopted. The melamine was extracted by precipitation of milk protein at its isoelectric point with dilute acids. Samples were purified by using membrane filter of 0.45m pore size, a known concentration of melamine standard solution was added to this filtrate and the absorbance was measured spectrophotometrically. The linearity curve was constructed for the concentration of melamine ranging from 3 - 8g/ml.The correlation co-efficient was found to be 0.9926. In this study 31 milk samples (raw milk and milk related product) were analysed by standard addition method. It was observed that all the 31samples were found to contain melamine residue. This is the first study to confirm the existence of melamine residue in pasteurized milk marketed in Tamilnadu.
Page(s) : 234-240
ISSN : 0975-9492
Source : Vol. 6, No.2