ISSN : 0975-9492


Open Access

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Title : New Analytical Methods and Their Validation for the Estimation of Analgin in Bulk and Marketed Formulations
Authors : Mona Bhalani, Dr. E.V.S. Subrahmanyam, Dr. A. R. Shabaraya
Keywords : analgin, ammonium molybdate, ferric chloride hexahydrate, 1,10- phenanthroline.
Issue Date : February 2015
Abstract :
Analgin is ampyrone sulphonate analgesic, antispasmodic and antipyretic which is most commonly given orally or parenterally to prevent and treat pain related to surgery or for the treatment of acute pain. Purpose: There was a need for the development of newer, simple, sensitive, rapid, accurate and reproducible analytical methods for the routine estimation of analgin in bulk and pharmaceutical dosage form since the reported method make use of highly sophisticated intruments. Methods: Two simple, sensitive, specific and validated methods (method A and method B) have been developed for the quantitative estimation of analgin in bulk and pharmaceutical dosage form. Method A is based on oxidation of analgin with ammonium molybdate to produce green colour chromogen which showed ?max at 682.8 nm. In method B, analgin oxidizes in presence of ferric chloride and subsequent complexation of resulting ferrous ion with 1,10- phenanthroline which absorbs at ?max at 511.2 nm. Reults: The linearity was found in concentration range of 5-30g/ml and 10-60g/ml for method A and method B respectively. The correlation coefficient was found to be 0.9997 and 0.9995 for method A and method B respectively. The methods were validated as per ICH guidelines. The LOD and LOQ for estimation of analgin were found as 0.1390, 0.4214 for method A and 0.1596, 0.4837 for method B respectively. Conclusions: Proposed methods were successfully applied for the quantitative estimation of analgin in marketed formulations.
Page(s) : 411-413
ISSN : 0975-9492
Source : Vol. 6, No.2