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ABSTRACT
| Title |
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Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Telmisartan and Hydrochlorothiazide in Bulk and Pharmaceutical Dosage Form |
| Authors |
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N. Mukuntha Kumar, Sumathi V rao, Konde Abbulu, B.Venkata Narayana, I. Sukumar |
| Keywords |
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Telmisartan, Hydrochlorothiazide, RP-HPLC, Simultaneous estimation, Method development and Validation. |
| Issue Date |
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October 2014 |
| Abstract |
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A simple, accurate, precise and rapid RP-HPLC method has been developed and validated for the simultaneous estimation of Telmisartan and Hydrochlorothiazide in bulk and fixed-dosage formulation. The separation was achieved on ACE 5 C18 (Length 150 mm × Diameter 4.6 mm Particle size 5 µm) column with gradient flow. The mobile phase at a flow rate of 1.5 mL/min consisted of Water: Acetonitrile: Orthophospharic acid (95:5:1 Mobile Phase A) and Water: Acetonitrile: Orthophospharic acid (5:95:1 Mobile Phase B) (Gradient ratio). The UV detection was carried out at 280 nm. The retention time of Hydrochlorothiazide and Telmisartan was found to be 4.19 and 9.12 min. respectively. The method has been validated for Specificity, Linearity, Accuracy, Precision and Robustness. The calibration curve for Telmisartan and Hydrochlorothiazide were linear from the range of 160.1-480.4 µg/mL and 25.2 - 75.7 µg/mL respectively. The mean recoveries obtained for Telmisartan and Hydrochlorothiazide were 100.1% and 99.8% respectively. The developed method was found to be Specific, accurate, Precise, Robust and rapid for the simultaneous estimation of Telmisartan and Hydrochlorothiazide in bulk Pharmaceutical Dosage Form. |
| Page(s) |
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646-654 |
| ISSN |
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0975-9492 |
| Source |
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Vol. 5, No.10 |
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