ISSN : 0975-9492


Open Access

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Title : Development of validated RP-HPLC method for the estimation of L-Dopa from Mucuna pruriens, its extracts and in Aphrodisiac formulation
Authors : Bhumika G. Rathod, Natvarlal M. Patel
Keywords : HPLC, L-Dopa, Mucuna Prurines, Atmagupta, Polyherbal formulation.
Issue Date : August 2014
Abstract :
In the present study, reversed phase HPLC method was developed for the estimation of L-Dopa from Mucuna Prurines, its extract and in Aphrodisiac formulation. HPLC analysis was performed on C18 column using a mixture of Water: Acetonitrile: Methanol containing 0.2 % Triethylamine PH adjusted to 3.3 as isocratic mobile phase at a flow rate of 1.0 ml per minute at detection wavelength of 280 nm. The method was validated for accuracy, precision, linearity, specificity and sensitivity in accordance with International conference on Harmonization guidelines. Validation revealed the method is specific, accurate, precise, reliable and reproducible. Good linear correlation coefficients (r2 > 0.999) was obtained for calibration plots in the range of 10 80 g/ml. Intraday and Interday RSD of retention times and peak areas were less than 2.0 %. Average Percent Recovery was 98.83 %. The method was successfully used for quantitative analysis of this marker compound in Polyherbal formulation.
Page(s) : 508-513
ISSN : 0975-9492
Source : Vol. 5, No.8