ISSN : 0975-9492
CODEN : IJPSQQ





INTERNATIONAL JOURNAL OF PHARMA SCIENCES AND RESEARCH


Open Access

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ABSTRACT

Title : Simultaneous Estimation of Phenylephrine HCl, Doxylamine Succinate and Dextromethorphan HBr in Soft Gelatin Capsule (Cough and Cold Preparation) by RP-HPLC
Authors : Mo.Salauddin A Shaikh, Harsh J Lakhani, Suddhasattya Dey, Prasanna K. Pradhan, Umesh M. Upadhyay
Keywords : Phenylephrine HCl, Doxylamine Succinate and Dextromethorphan HBr, RP-HPLC, Validation
Issue Date : May 2014
Abstract :
A RP-HPLC methods were developed and validated for simultaneous estimation of Phenylephrine HCl (PE), Doxylamine Succinate (DOX) and Dextromethorphan HBr (DEX). The separation was achieved on a Princestone ODS C18 (250mm X 4.6 mm i.d., 10 m particle size) with an Isocratic system of Phosphate Buffer (10 mM) : Methanol : Acetonitrile (pH 4) in the ratio of 70:25:5 v/v/v. The retention time for PE, DOX and DEX was obtained as 5.125 min, 10.419 min and 2.661 min respectively with a flow rate of 1.0 ml/min at detection wavelength 215 nm. The linearity of the proposed method was investigated in the range of 25 -125 g/ml, 31.25 - 156.25 g/ml and 50 - 250 g/ml for PE, DOX and DEX respectively. The developed method was validated as per ICH guideline and found to be satisfactory.
Page(s) : 218-225
ISSN : 0975-9492
Source : Vol. 5, No.5