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ABSTRACT
| Title |
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PHYLOGENETIC ANALYSIS AND HOMOLOGY MODELLING OF LACCASE GENE OF FUSARIUM OXYSPORUM Lcc2 |
| Authors |
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S.Arul Diana Christie, S.Shanmugam |
| Keywords |
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Homology Modelling, Laccase, Lcc2 gene, F.oxysporum. |
| Issue Date |
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December 2012 |
| Abstract |
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Morphological identification of fungi is the first and the most difficult step in the identification process. This is especially true for Fusarium species. Fusarium has a cosmopolitan distribution. Fusarium sp was isolated from Anamalai Hills region and Subjected to Morphological identification using standard microbiological methods. Genotypic identification was carried out by PCR amplification and partial sequencing of the 16S rRNA for the confirmation of morphological identity. The rDNA sequence of 16S ribosomal RNA gene was amplified by PCR and the PCR product .The fungus, was bi directionally sequenced using forward and reverse primers. Using BLAST Tool the sequence was searched for the similarity matches .F.oxysporum shows maximum similarity. The Gene of the species was obtained from Uniprot. Since the unavailability of the 3D structure of Fusarium Laccase gene this attempt was carried out and Homology modeling was performed using Modeller Tool. |
| Page(s) |
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571-582 |
| ISSN |
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0975-9492 |
| Source |
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Vol. 3, No.12 |
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