ISSN : 0975-9492
CODEN : IJPSQQ





INTERNATIONAL JOURNAL OF PHARMA SCIENCES AND RESEARCH


Open Access

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ABSTRACT

Title : Chromosomal aberrations induced by Glycidol in Allium cepa L root meristem cells
Authors : N.Panneerselvam*, L.Palanikumar, S.Gopinathan
Keywords : Glycidol, Allium cepa L, cytotoxicity, chromosomal aberrations, metaphase
Issue Date : February 2012
Abstract :
Glycidol is used as a stabilizer in the manufacture of vinyl polymers and natural oils and as an intermediate in the synthesis of glycerol, glycidyl ethers, and amines. It is also used as an alkylating agent, demulsifier, and dye-leveling agent and for sterilizing milk of magnesia. Glycidol is an alkylating agent which reacts readily with glutathione; it causes a decrease in glutathione content in rat liver, probably reflecting its binding to glutathione. It is a suspected reproductive toxicant and exposure to it has the potential to negatively affect the human reproductive system. The study investigated the cytotoxic effects of glycidol in Allium cepa L root meristem cells. Different concentrations of glycidol ranging 10, 20, 40 and 50g/ml for 1, 2, 3 and 5h were treated with root meristem cells of Allium cepa L. Fuelgen squash technique was used to determine the aberrations in root tip cells. For each concentration 3000 well-spread cells were scored and cytological abnormalities such as break, gap, exchange, multiple breaks and chromosome fragments were observed. One way analysis of variance was used the study the significant different control and treatment exposed root tip cells. Significant difference between control and glycidol exposed cells were observed. Increasing concentrations increased the number of chromosomal aberrations.
Page(s) : 300-304
ISSN : 0975-9492
Source : Vol. 3, No.2