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ABSTRACT
| Title |
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Inhibition of Pro-oxidant induced DNA Damage in isolated human peripheral lymphocytes by methanolic extract of Guduchi (Tinospora cordifolia) leaves. |
| Authors |
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Chandrashekar S, Umesha S, Chethan Kumar M, Chandan S |
| Keywords |
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Tinospora cordifolia, DNA fragmentation, Lymphocytes, Free radicals, pro-oxidant DNA protectant Activity. Reactive oxygen species |
| Issue Date |
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November 2010 |
| Abstract |
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The present preliminary investigation reveals the anti-oxidant effects of Guduchi (Tinospora cordifolia) leaves. Dried and powdered leaves of Tinospora cordifolia was subjected to methanol extraction, and total phenols, sugars and proteins were determined. DNA fragmentation was carried out using isolated human peripheral lymphocytes and Calf thymus DNA as a model system. The results showed that the methanol extract of Tinospora cordifolia is rich in total polyphenols (>3 mg/g) in comparison to sugars (<2 mg/g) and protein (<1 mg/g). DNA fragmentation was measured by diphenylamine method. The polyphenols rich fraction of Tinospora cordifolia leaves were found to offer 64% protection on Fe:As (10:100µmole) induced DNA fragmentation, where as BHA offered protection by 88% and was found to have direct correlation between the total polyphenols and its protective against pro-oxidant induced DNA fragmentation. The in-vitro anti-oxidant activity of Tinospora cordifolia justifies the medicinal use of this plant. |
| Page(s) |
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445-450 |
| ISSN |
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0975-9492 |
| Source |
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Vol. 1, No.10 |
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