ISSN : 0975-9492
CODEN : IJPSQQ





INTERNATIONAL JOURNAL OF PHARMA SCIENCES AND RESEARCH


Open Access

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ABSTRACT

Title : A NEW RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF ORLISTAT IN BULK AND PHARMACEUTICAL DOSAGE FORMS
Authors : N.Sreekanth, CH.Babu Rao, K.Mukkanti
Keywords : Orlistat, HPLC, Isocratic, validation.
Issue Date : July 2010
Abstract :
A simple, accurate and rapid RP-HPLC method has been developed for the estimation of Orlistat (ORL) in bulk and pharmaceutical dosage forms using a C 18 column 150 x 4.6 mm i.d, 3.5?m particle size in isocratic mode, with mobile phase comprising of acetonitrile, water and phosphoric acid in the ratio of 85:15:0.5 (v/v/v). The flow rate was 1ml/min and detection was carried out by UV detector at 205nm. The retention time for ORL was found to be 3.79 min. The proposed method has permitted the quantification of ORL over linearity in the range of 6-60g/ml and its percentage recovery was found to be 99.78-100.27%. The % RSD of intra day and inter day precision were found 0.49% and 0.57%, respectively.
Page(s) : 251-257
ISSN : 0975-9492
Source : Vol. 1, No.6