ISSN : 0975-9492


Open Access

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Title : In-Vitro Anti fungal Screening of Embelia Ribes Plant Extract through EUCAST Method
Authors : Sanjesh G Rathi, Vaidhun H Bhaskar, Paras G Patel
Keywords : Embelin, Embelia ribes, EUCAST method, TLC
Issue Date : March 2010
Abstract :
Aim of the present study was to investigate the antifungal activity of the Embelia ribes plant extracts using standard in vitro antifungal susceptibility test method like EUCAST (European Committee on Antimicrobial Susceptibility Testing M27-A2 Protocol).Antifungal screening of Embelia ribes not studied in detail and not extended to the different spectrum of fungal which are causing human diseases. Thus different types of extracts were prepared using different solvents and TLC characterized. Assays were performed in 96 well plates and detection was carried out with colorimetric plate reader at 530nm.To obtain the MIC50 with the help of the graph pad prism software.The petroleum ether extract, solvent ether extract and methanol extract had low MIC50 values against the Candida species than the other species. Solvent ether extract and petroleum ether extract were highly effective against the c.albicans (MTCC NO 183) with the 65 mg/L and 32mg/L MIC50 values. The methanol extract were more active against c.albican (MTCC NO 183) species with 300-500 mg/L MIC50 value. The solvent ether extract, petroleum ether extract, methanol extract, potassium embelate and Embelin have reported the MIC50 values in range of 800-1600 mg/L against c.tropicalis (MTCC NO 184) and c.parapsilosis (MTCC NO 1744). The petroleum ether extract and potassium embelate was found to have MIC50 between range 300-700 mg/L against c.parapsilosis (MTCC NO 1744) and a.fumigatus (MTCC NO 2550) .Other extracts required higher concentrations against c.parapsilosis and a.fumigatus. Water extract was found to have MIC50 values greater than 2000 mg/L against all fungus. Most of the results for the Embelin could not obtain by EUCAST method due to having higher fluctuation in results.
Page(s) : 134-138
ISSN : 0975-9492
Source : Vol. 1, No.2