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ABSTRACT
| Title |
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Development and Validation of a Stability indicating HPLCmethod for the Determination of Lamivudine in Bulk and Pharmaceutical dosage form. |
| Authors |
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S.V.Saibaba, Dr.P.Shanmuga Pandiyan |
| Keywords |
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Lamivudine,RP-HPLC,Validation,Stability indicating, Stress condition |
| Issue Date |
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Dec 2016 |
| Abstract |
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A simple,selective, precise and accurate RP-HPLC method was developed and validated for the estimation of lamivudine in bulk and pharmaceutical dosage form. The determination was carried out on IntersilC-18 (250 X 4.6mm, 5µm) column using a mixture of Acetonitrile,water (50:50% v/v) as mobile phase with a flow rate of 1.0ml/min. The estimation was carried out at 270nm.The method was validated for linearity, accuracy, precision, specificity and robustness as per ICH norms. The retention time of the lamivudine was 2.6 min. The method shows linear responses in the concentration range of 40- 120 µg/ml. with correlation coefficient (r2) of 0.999. This method can be useful for the estimation of lamivudine in its pure and tablet dosage forms. |
| Page(s) |
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471-476 |
| ISSN |
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0975-9492 |
| Source |
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Vol. 7, No.12 |
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