Abstract of INTERNATIONAL JOURNAL OF PHARMA SCIENCES AND RESEARCH (IJPSR)

ABSTRACT

Title

Radio protective herbal formulation against UV exposure

Authors

Manish Kumar, Mohini Agarwal, Muktinath Kumar, Kumud Bala

Keywords

Radio protective agents, free radical scavenging activity, herbal radioprotectors, ionising radiation,antioxidant.

Issue Date

October 2016

Abstract

Exposure to high amounts of ionizing radiation or other radiations causes damages to the hematopoietic, gastrointestinal or central nervous systems. Due to the damage caused by ionizing radiation and its impact on the human life, there is a need to develop an effective and non-toxic radioprotectent. Various herbs has been mentioned as radioprotectant in the Ayurvedic treatment with negligible side effects. Some of the Herbal extracts with therapeutic benefit may work as a potential radioprotectent in intentional as well accidental radiation. This study aims at finding out the efficacy of organic Berberine and Selenium as possible radioprotectant. Experiment has been performed on E coli DH5α at different durations (at 15, 30 and 60 minutes) of radiation (UVC radiation from 100nm-280nm) and at various concentrations of Berberine (drug D1) and Bereberine+Selenium (drug D2) treatment. Analysis of DH5α was done by spread plate method, colony counting after drug D1 and D2 treatment, DNA damage and protein expression profile with and without UV irradiation. Biochemical assays were also performed to asses GST, GSH and CAT activity. Results after 30 minutes of UV exposure with drug D1 and D2 treatment were analysed. It showed that 20µg/ml of drug D1 has radioprotective activity as per spread plate method, however drug D2 at the concentration of 40μg/ml has shown the colony enhancement as well as DNA protectant ability by keeping the DNA intact on the gel as compared to the UV exposed control plate, which has shown DNA shearing after UV exposure. CAT was found to be highest in drug D2 at the concentration of 40μg/ml i.e. 0.7umol/min/ml after 30 minutes of exposure. GST and GSH were also found highest in drug D2 which was in the concentration of 20ug/ml i.e. 0.021μmol/min/ml and at the concentration of 60μg/ml which was 0 .0185μmol/min/ml respectively. Later a cream based formulation of the drug D1 and D2 was prepared using 2:1 ratio of the drugs. Formulation stability was assessed by Organoleptic evaluation. It has been observed through the experiment that drug D2 at 40μg/ml in the ratio of 2:1 (Berberine+selenium) was able to provide necessary protection against UV radiation.

Page(s)

373-381

ISSN

0975-9492

Source

Vol. 7, No.10