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ABSTRACT
Title |
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PRELIMINARY PHYTOCHEMISTRY AND INVITRO CYTOTOXIC ACTIVITY OF THE LEAVES OF Asparagus racemosus Willd., (Liliaceae) |
Authors |
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K. Durai Prabakaran, R.Vadivu, N.Jayshree |
Keywords |
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Asparagus racemosus, phytochemistry, invitro, MTT assay. |
Issue Date |
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April 2015 |
Abstract |
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Asparagus racemosus Willd.(Shatavari) is a well known plant in Ayurvedic systems of medicine. ‘Shatavari’ is a reputed classical drug and said to possess therapeutic properties as Rasayana drugs of Ayurveda. The present study is aimed at the development of phytochemical parameters and invitro cytotoxic activity of various extracts of the leaves of Asparagus racemosus plant. The plant material was successively extracted with solvents of increasing polarity namely n-Hexane, chloroform, ethyl acetate and ethanol in a soxhlet extractor. Preliminary phytochemical tests, fluorescence analysis, thin layer chromatography and high performance thin layer chromatographic studies were carried out for all the extracts. Chloroform, ethyl acetate and ethanol extracts were screened for invitro cytotoxic activity by MTT assay using EAC (Ehrlich’s Ascites Carcinoma) cell line. The preliminary phytochemical analysis of various extracts of the leaves showed the presence of carbohydrates, tannins, phenolic compounds, sterols and flavonoids. The chloroform and ethyl acetate extracts showed significant cytotoxic activity when compared to ethanol extract against the above mentioned cancer cell line. |
Page(s) |
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743-748 |
ISSN |
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0975-9492 |
Source |
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Vol. 6, No.4 |
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