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ABSTRACT
| Title |
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Effect of Ethanol Leaf Extract of Piliostigma thonningii on Liver Function indices following Pefloxacin induced Toxicity in Wistar Albino Rats. |
| Authors |
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Dasofunjo K, Alagwu.E.A, Okwari O. O, Ipav S.S, Ezugwu, H.C, Onazi A.C |
| Keywords |
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DNA gyrase, Hepatic injury, Hepatoprotective, Pefloxacin, Piliostigma thonningii, |
| Issue Date |
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April 2015 |
| Abstract |
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Purpose: Pefloxacin is a broad spectrum antibiotic that is active against Gram-negative bacteria by inhibiting DNA gyrase. This present research determines the effect of ethanol leaf extract of Piliostigma thonningii following Pefloxacin induced toxicity in wistar albino rats. Methodology: Twenty (20) male wistar albino rats ranging from 180-200g were acclimatized to laboratory conditions for 7days, following which they were randomly assigned into 4 groups A, B, C and D of five animals each. Group B was administered 0.5 ml of ethanol extract via oral route corresponding to 200mg/Kg/body weight, group C was administered with Pefloxacin (400mg/5ml) only, group D was co administered with ethanol leaf extract of P. thonningii and Pefloxacin (1:1) while group A. (control) received 0.5ml of distilled water orally. The rats was housed in wooden cages. The animal room was ventilated and kept at room temperature and relative humidity of 29°c and 40-70% respectively with 12 hours natural light-dark cycle and were allowed free access to food and water ad libitum. Good hygiene was maintained by constant cleaning and removal of faeces and spilled from cages daily. Rats in all groups were sacrificed 24 hours after the experimental periods of 21days of oral administration. Results: The result reveals a significant (P<0.05) increase in serum albumin in all the experimental groups when compared with the control. Likewise, serum globulin depicts a significant increase (P<0.05) while groups treated with Pefloxacin and mixture of Pefloxacin and ethanol leaf extract of Piliostigma thonningii showed significant (P<0.05) decrease when compared with the control. Except for group B which reveals a significant (P< 0.05) increase in serum bilirubin, Pefloxacin and mixture of Pefloxacin and ethanol leaf extract of Piliostigma thonningii showed a significant decrease when compared with the control. The serum AST concentration showed a significant (P<0.05) decrease when compared with the control. Liver AST and ALP reveals a significant decrease in groups administered with ethanol leaf extract of Piliostigma thonningii and mixture of Pefloxacin and ethanol leaf extract of Piliostigma thonningii while groups administered with the drug showed a significant (P<0.05) increase when compared with the control. Likewise, the Liver ALP showed a significant (P<0.05) increase in groups treated with the extract and drug alone while groups treated with both the extract and Pefloxacin showed a significant decrease (P<0.05) when compared with the control. Conclusion: The alterations on the liver functional indices studied suggest that ethanol leaf extract of p. thonningii possess a hepato protective effect, but with the evidence of hepatic injury/assault in groups treated with Pefloxacin but was significantly ameliorated in groups co- administered with the drug and the extract. |
| Page(s) |
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783-789 |
| ISSN |
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0975-9492 |
| Source |
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Vol. 6, No.4 |
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