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ABSTRACT
| Title |
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RP-HPLC Method for Simultaneous Estimation of Enalapril Maleate and Chlorthalidone in Synthetic Mixture |
| Authors |
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VISHWA M. DAVE, Dr. DILIP G. MAHESHWARI |
| Keywords |
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Enalapril Maleate, Chlorthalidone, RP-HPLC method |
| Issue Date |
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April 2015 |
| Abstract |
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A simple, accurate, precise and rapid RP-HPLC method has been developed for simultaneous estimation of Enalapril Maleate and Chlorthalidone in synthetic mixture. The mobile phase used consisting of Phosphate buffer: Acetonitrile: Methanol (65:25:10 v/v/v) was used at the flow rate of 1.0 mL/min and detection was carried out at 210 nm. The separation was achieved using Hypersil BDS C18 (250 x 4.6mm, 5 µm ). Linearity was obtained in the range of 5-15 µg/ml and 12.5-37.5 µg/ml for Enalapril Maleate and Chlorthalidone respectively with R2 value 0f 0.999 for Enalapril Maleate and 0.998 for Chlorthalidone. Retention time for Enalapril Maleate and Chlorthalidone is 7.749 and 4.247 respectively. The recovery studies were found in between 99.83-100.88 for Enalapril Maleate and for Chlorthalidone 99.33-101.11 with relative standard deviation less than 2. The method was validated as per the ICH guidelines Q2(R1). |
| Page(s) |
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666-673 |
| ISSN |
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0975-9492 |
| Source |
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Vol. 6, No.4 |
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