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ABSTRACT
Title |
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EVALUATION OF ANTIMICROBIAL ACTIVITIES PROPERTIES OF BASELLA RUBRA METHANOLIC EXTRACTS ON SELECTED MICROORGANISMS. |
Authors |
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Krishana Priya, Ankur Gupta, Surabhi Mahajan, R.K.Agnihotri, Rajendra Sharma |
Keywords |
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Antimicrobial activity, Disc Diffusion method, Basella rubra, MIC |
Issue Date |
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February 2015 |
Abstract |
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In vitro antibacterial activity of Basella rubra commonly used by the Indian community was analyzed in this study. Traditional uses of Basella rubra include general tonics and over-the-counter medications used to treat specific conditions or diseases. The present study examined the antibacterial activity of Basella rubra extract using the disk diffusion method as part of the process of understanding the chemistry, toxicity and efficacy of Basella rubra extract. Methanolic extracts of the herb were examined using a standard antimicrobial disk diffusion method. Extracts were tested against E. coli (MTCC No. 1652), Pseudomonas aeruginosa (MTCC No. 424), Bacillus subtilis (MTCC No. 2393) and Aspergillus flavus (MTCC No. 277) bacteria. The inhibition zones were significantly different in each plant extract. The methanolic extract of leaves showed activity with zone at (MIC of 3.125 mg/ml) against E.coli and Pseudomonas aeruginosa followed by Bacillus subtilis (MIC of 6.25 mg/ml), Aspergillus flavus (MIC of 12.5 mg/ml) while methanolic extract of stem showed activity with zone at (MIC of 3.125 mg/ml) against E.coli, followed by Pseudomonas aeruginosa (MIC of 6.25 mg/ml), Bacillus subtilis (MIC of 12.5 mg/ml), Aspergillus flavus (MIC of 25 mg/ml). The phytochemical components of the methanolic extracts of the leaf and stem of Basella rubra showed the presence of different compounds such as leaf showed presence of steroids and carbohydrates, while stem extract showed presence of Tannin flavonoids and steroids. This study serves as basis for further research on Basella rubra extract. |
Page(s) |
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334-336 |
ISSN |
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0975-9492 |
Source |
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Vol. 6, No.2 |
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