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ABSTRACT
Title |
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Enhanced Production of Tannase from Redgram Husk Using Response Surface Methodology |
Authors |
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S.K.Mohan, T.Viruthagiri, C.Arunkumar |
Keywords |
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Tannase, Response Surface Methodology, Aspergillus flavus, Submerged fermentation |
Issue Date |
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December 2013 |
Abstract |
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PURPOSE:To Study the Statistical Optimization of fermentation conditions for maximum production of tannase enzyme by Aspergillus flavus (MTCC 3783), using redgram husk as a substrate was studied using the Response Surface Methodology.
METHOD:The present study was done via two steps. At first to study the maximum production of tannase enzyme by Aspergillus flavus, using redgram husk as a substrate followed by Statistical Optimization of Process parameters using Response Surface Methodology.
RESULTS:The optimum conditions were tannic acid concentration, 2.8 % (w/v); fermentation period, 95 h; temperature, 34ºC; and pH 5.65. Higher value of the regression coefficient (R2 = 0.9806) indicates excellent evaluation of experimental data by second-order polynomial regression model. The RSM revealed that a maximum tannase production of 143.5 U/ml was obtained at the optimum conditions.
CONCLUSIONS:The Response Surface Methodology using Central Composite Design was effectively applied for the production of tannase from Aspergillus flavus, using redgram husk as a substrate in submerged fermentation. |
Page(s) |
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144-153 |
ISSN |
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0975-9492 |
Source |
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Vol. 4, No.12 |
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