|
|
ABSTRACT
| Title |
: |
Evaluation of single nucleotide polymorphism C825T in the GNB3 gene with the development of type 2 diabetes of the South Indian population |
| Authors |
: |
S. Chandrasekaran, S. Sivani, D. Sudarsanam |
| Keywords |
: |
BsaJI, G-protein, GNB3, PCR, RFLP, SNP, type 2 diabetes mellitus |
| Issue Date |
: |
January 2012 |
| Abstract |
: |
Diabetes mellitus, a widespread metabolic disorder, is caused by a deficiency of insulin or by a resistance to insulin and characterized by hyperglycemia. G-proteins, a class of signaling molecules, are involved in the signaling pathway of insulin. Any defect in the G-proteins leads to a defect in the GNB3 gene, coding for a Gprotein that has been associated with the development of type 2 diabetes with related complications like hypertension and obesity. 70 subjects with type 2 diabetes and 70 control subjects were genotyped for the SNP using gene-specific PCR amplification and restriction digestion using the enzyme BsaJI, which cleaves the wide-type allele and not the mutant allele. The frequency of occurrence of the mutant and the wild-type alleles were statistically analyzed for significant relations. The statistical analysis with a p-value of 0.469 denotes the above, the ‘acceptable’ error rate. Therefore, the SNP C825T int eh GNB3 gene is not significantly associated with type 2 diabetes mellitus in the South Indian population. |
| Page(s) |
: |
249-257 |
| ISSN |
: |
0975-9492 |
| Source |
: |
Vol. 3, No.1 |
|
Copyright to ENGG Journals Publications
