| Abstract |
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Background:
Cell death is the main component of many response patterns of living tissues to xenobiotics including the organochlorine insecticide, dichlorodiphenyl- trichloroethane (DDT). One of the possible ways to ameliorate this response is through interference with the process of oxidative stress which can be fulfilled by many candidate substances like green tea.
Objective:
The present study is designed to evaluate the influence of simultaneous administration of Green Tea, a protective and antioxidant agent, on the status of lipid peroxidation products (MDA) and glutathione (GSH) in liver tissue with measuring the activity of serum liver enzymes (AST, ALT and ALP) in rats treated with a toxic dose of DDT.
Methods:
Twenty four white Albino rats of both sexes, weighing 200- 250gm were used in this study; these animals classified into four groups as follow:
Group I: Six rats received single oral dose of corn oil (3 ml) using gavage tube. The animals were anesthetized by ether and sacrificed by decapitation 24 hr after administering corn oil dose .This group served as negative control.
Group II: Six rats received single oral dose of DDT 100mg/kg (dissolved in corn oil) by gavage tube. The animals were anesthetized by ether and sacrificed by decapitation 24hr after administering DDT dose. This group served as positive control of DDT- induced hepatic toxicity.
Group III: Six rats were utilized to study the possible protective effects of AGTE against DDT- induced liver damage which received oral dose of green tea ( 1.25% AGTE as their sole source of drinking fluid) given daily by feeding bottle for 7-days ( started 7-days prior to and during the period of treatment with DDT , at day 7 DDT dose (100 mg / kg /day)was given, then the animals were anesthetized by ether and sacrificed by decapitation 24hr after administering DDT dose) .
Group IV: Six rats were utilized to study the possible protective effects of AGTE against DDT induced liver damage which received oral dose of green tea (10% AGTE as their sole source of drinking fluid) given daily by feeding bottle for 7-days( started 7-days prior to and during the period of treatment with DDT, at day 7 DDT dose (100 mg / kg /day)was given, then the animals were anesthetized by ether and sacrificed by decapitation 24hr after administering DDT dose) .
At the end of treatment all animals were sacrificed to measure the oxidative stress markers, malondialdehyde (MDA) contents and glutathione (GSH) levels in liver tissue homogenate. Serum was obtained for the assay of liver enzymes activity [aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP)].
Results:
In this study, we investigated the changes in the content of end product of lipid peroxidation (MDA) and the level of glutathione (GSH) which are critical determinants of tissue susceptibility to oxidative damage in liver tissue homogenate as a result of DDT administration.
Rats treated with a single oral dose 100mg/kg/day of DDT showed (48%) elevation of MDA contents and (42%) depletion of GSH levels in liver tissue homogenate compared to control group(p<0.05) .
Rats orally treated with different concentrations (1.25% and 10%) of AGTE for 7 days prior to and during DDT treatment , showed a significant decrease in MDA contents in liver tissue homogenate (30% and 31.6%) respectively and a significant increase in GSH levels in liver tissue homogenate (59% and 48.3%) respectively compared to DDT-treated group(p<0.05) , also showed a non significant compared to control group(p>0.05) .
Rats treated with a single oral dose 100mg/kg/day of DDT produced a significant increase in the serum levels of AST, ALT and ALP(77.5% , 182% and 68%)compared to control groups(p<0.05) indicating a considerable hepato-cellular injury .
Rats treated with different oral concentrations ( 1.25% and 10%) of AGTE for 7 days prior to and during DDT treatment produced a significant decrease in the levels of serum AST , ALT and ALP[(46.2% , 72.7% ,53%) and (44.2% , 64.2% and 45.4%) ] compared to DDT treated group (P<0.05) .
Conclusion:
According to the results obtained in this study we can conclude that Green Tea have the ability, through a mechanism related to it's antioxidant property to provide protective effects against DDT-induced hepatotoxicity .By [the reducation of hepatic oxidative stress (reduction in MDA contents with the elevation of GSH levels) with subsequent improvement in liver functions manifested by reduction in the serum levels of (AST , ALT and ALP)], that improving the damage in liver tissue and makes it a good candidate to be tried clinically in this respect. |
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