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ABSTRACT
Title |
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Antimicrobial Property of Piper betel Leaf against Clinical Isolates of Bacteria |
Authors |
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Arani Datta, Shreya Ghoshdastidar, Mukesh Singh |
Keywords |
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antimicrobial activity, Piper betel, phytochemical, pathogenic isolates |
Issue Date |
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March 2011 |
Abstract |
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The leaves of Piper betel (locally known as Paan) have long been in use in the Indian local system of medicine for its antioxidant and antimicrobial properties. In the present work, the antimicrobial activity of ethanol extract of Piper betel leaves was evaluated against human pathogenic bacteria (both gram-positive and gram-negative). The leaf powder was subjected to phytochemical screening and was found to contain carbohydrate, protein, polyphenolic compounds, flavonoid, alkaloids and total antioxidant. The ethanol extract showed strong free radical scavenging activity as seen by DPPH model. The extract confirmed significant antimicrobial activity against all bacterial strains tested. The effect of the extract was almost proportional to the concentration of the extract tested. The MIC for the bacterial strains was in the range of 25 µg to 40 µg. Concurring with the disc diffusion results, the MIC of the Proteus vulgaris was found to be least 25 µg while for Staphylococcus aureus it was approximately 40 µg. Time-kill kinetics of the ethanol extract treated bacterial strains demonstrated similar results, showing decline in the growth curve after six hour in most of the strains. Crude ethanol extract of Piper betel showed strong antimicrobial activity against the tested pathogenic bacterial strains. The results also indicate that scientific studies carried out commonly use herbs having traditional claims of effectiveness might warrant fruitful results. |
Page(s) |
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104-109 |
ISSN |
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0975-9492 |
Source |
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Vol. 2, No.3 |
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