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ABSTRACT
| Title |
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STABILITY INDICATING LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF OLMESARTAN MEDOXOMIL AND AZELNIDIPINE IN COMBINED TABLET DOSAGE FORM |
| Authors |
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Raveendra Babu Ganduri, Jayachandra Reddy Peddapapireddigari, Himabindu Vurimindi, Rampraksah |
| Keywords |
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olmesartan medoxomil, azelnidipine, RP-HPLC, stability-indicating, validation. |
| Issue Date |
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June 2014 |
| Abstract |
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A stability indicating RP-HPLC method for the simultaneous determination of olmesartan medoxomil (OLM) and azelnidipine from combined tablet dosage form was developed. The separation was accomplished on Inertsil 3V (4.6 mm X 100 mm; particle size 3 µm) column using a mobile phase consisting of potassium dihydrogen phosphate buffer (pH adjusted to 3.0 with orthophosphoric acid) and acetonitrile in gradient elution mode. The analytes were monitored by a photo diode array (PDA) detector set at 255 nm and the flow rate was kept at 2.0 mL min-1. The retention time for olmesartan medoxomil and azelnidipine were 3.148 and 3.704 respectively. Linearity was observed in the concentration range of 10-60 µg/mL for olmesartan medoxomil and 4-24 µg/mL azelnidipine. Both the drugs were subjected to acid, alkali and neutral hydrolysis, oxidation, dry heat and photolytic degradation. The degradants were well resolved from the pure drugs. The method could be used for simultaneous determination of olmesartan medoxomil and azelnidipine in bulk and combined dosage form. |
| Page(s) |
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275-282 |
| ISSN |
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0975-9492 |
| Source |
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Vol. 5, No.6 |
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